Date of Completion

2023

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Strawberry, Methicillin-Resistant Staphylococcus aureus, Kirby-Bauer Method, Minimum Inhibitory Concentration, Minimum Bactericidal Concentration

Abstract

Methicillin-Resistant Staphylococcus aureus (MRSA) is a well-known multi-drug resistant bacteria that use vancomycin and daptomycin to treat this emerging health concern. An extract obtained from Fragaria x ananassa (strawberry) was assessed for its antimicrobial activity against ATCC® 43300 Methicillin-Resistant Staphylococcus aureus in studying alternatives for the antibiotics used in treating MRSA. Different concentrations of Fragaria x ananassa (100%, 75%, 50%, 25%) were assessed against ATCC® Methicillin-Resistant Staphylococcus aureus. According to the results, only 100% and 75% concentrations of the extract in the Kirby-Bauer Method inhibited MRSA but were not satisfactory to the American Society for Microbiology standards in 2016. Furthermore, Minimum Inhibitory Concentration (MIC) was performed with a 1:2 ratio, with decreasing Fragaria x ananassa extract concentrations. The results suggested that the significant inhibitory effect against

MRSA was not significant. Consequently, Minimum Bactericidal Concentration (MBC) was not performed due to the lack of at least two dilutions representing the MIC (Daly et al., 2017). Therefore, Fragaria x ananassa extract cannot be used as an antimicrobial agent. Future studies of the same topic are recommended to apply optimal storage in preserving its phytochemical properties, different serial dilution factors in MIC, perform the potential synergistic antimicrobial activity with other fruit extracts against MRSA, freeze drying or oven drying the samples in the extraction process, using the fruit extract as a selective culture medium to isolate MRSA and test the fruit extract if it can inhibit the enzymes that degrade the methicillin.

First Advisor

Vincent Mark G. Tandog

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