Title

Efficacy of the ethanolic extract of tar (colocasia esculenta) leaves as an antioxidant

Date of Completion

2017

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Antioxidants, Plant Extracts, Ethanol, Tars

Abstract

This study employed an experimental research design. There were various tests that were utilized to determine the antioxidative activity of Taro leaves . First, the plant was acquired from Silang, Cavite. Then, it was authenticated at the Bureau of Plant Industry. After the plant authentication, it was dried in preparation to the Soxhlet extraction. This was the main method in obtaining the ethanolic extract of the taro leaves. for this extraction, ethanol was the solvent of choice. Second, the ethanolic crude extract was subjected for phytochemical analysis. It determined the phytochemical components that give the Taro leaves its antioxidant property. Third, DPPH Radical Scavenging Assay was performed to confirm the total antioxidant activity of the ethanolic crude extract. After the initial screening tests for the antioxidant component of the Taro leaves, the experiment proceeded to apply its effectiveness in preventing oxidation by performing Hemolysis Assay. The results of the study showed that taro leaves contains polyphenols and flavonoids as displayed in the results of the phytochemical screening. The concentrations of taro leaves specifically 1000, 500, 250, 125, 62.5 uL are all comparable with the antioxidant activity of ascorbic acid. The study concluded that the hemolytic assay shows that the higher the concentration of the extract, the higher the inhibition.

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