Isolation, purification and partial characterization of Fagraea racemosa leaf extracts

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Bachelor of Science in Biochemistry




The air-dried leaves of Fagraea racemosa were sequentially extracted in three different organic solvents with increasing polarities such as petroleum ether (PE), dichloromethane (DCM) and ethanol. then, the DCM crude extract was purified by using silica gel column choromatography and was able to obtain two pure isolate compounds. These pure isolates showed characteristics of terpenes with the use of spectoscopic techniques via UV-Vis and FT-IR. Moreover, the premilinary phtochemical screening of PE, DCM and EtOH crude extracts from leaves of Fagraea racemosa revealed the presence of alkaloids, flavonoids, glycosides, phenols, saponins and terpenes. Due to presence of these phytochemicals, the crude extracts were screened for its antbacterial activity using Kibry Bauer disc diffusion method to evaluate its susceptibility against representative gram-positive and gram-negative bacteria. Based on the results, DCM and EtOH crude extracts have exhibited weak inhibition against gram-negative bacteria whereas, only DCM crude extract had weak inhibition against gram-positive bacteria. Thus, this was the first study for antibacterial activity of Fagraea racemosa leaf extract.

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