Title

Isolation and partial characterization of proteins from the seeds of Lantana camara L. exhibiting antibacterial activity

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Anti-bacterial Agents.

Abstract

Antibacterial proteins (ABPs) were isolated from the seeds of Lantana camara L. The protein extracts obtained were crude and dialyzed crude protein extracts. Both of these extracts were subjected to preliminary phytochemical test - biuret, ninhydrin and xanthoproteic tests for present of proteins. Also, these were partially characterized using SDS-PAGE, wherein prominent bands were observed, confirming the present of proteints. The concentration of the isolated proteins was also determined using the Bradford Standard Assay (BSA) and yielded 0.4270 mg.mL of protein present for every 50 uL of crude protein extract, and 0.1418 mg/mL protein present for every 50 uL of dialyzed crude protein extract. Staphylococcus aureus and Escherichia coli using the Kirby Bauer method and performed in triplicate analysis. Each protein extract were subjected to different concentrations. The zones of inhibition of each protein extract with corresponding cocentration were measured, confirmed antibacterial activity against the bacteria used. It may be recommended for these ABPs from the seeds of Lantana camara L. to be subjected for further characterization for future medicinal and economic use.

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