Green synthesis of silver nanoparticles using leaf extracts from Artocarpus doratissimus blanco against methicillin-resistant Staphylococcus aureus (MRSA)

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Drug Resistance, Bacterial.

Abstract

In this study, it aims to synthesize silver nanoparticles through green synthesis; evaluate its antimicrobial efficacy against an antibiotic resistant microorganisms; and to be able to characterize the nanoparticle in terms of its shape and size. A certain concentration of AgNO3 is reduced using varying volume of extract coming from the leaves of A. odoratissimus Blanco; the solution was then incubated at varying time. For every time interval, the AgNPs solution was then incubated at varying time. For every time interval, the AgNPs solution was subjected to UV-Vis spectrophotometer; the solution with the highest peak signifies the AgNPs synthesized at the optimal condition. The solution with the maximum production of AgNPs was observed at 6mL of extract added and was incubated for 42 hours in which the absorbance recorded was 2.3. The sysnthesized AgNPs at optimum conditions was then used to test its antimicrobial activity against a resistant bacteria (MRSA); the solution was also sent fro further characterization using FTIR and SEM. FTIR analysis showed that the sharpest peak present was around 1384 cm-1 which signifies a C-O stretching. The SEM imaging showed the nanoparticle sizes ranged at 40-60nm. The synthesized AgNPs yielded a moderate inhibition against MRSA with an average of 14.6 mm zone of inhibition.

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