Title

Antibacterial activity and phytochemical screening of musa acuminata leaf extract

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Antibacterial Agent.

Abstract

The primary objective of the study was to determine the secondary metabolites through phytochemical screening and determine the antibacterial property by using MIC of the ethanolic leaf extracts of Musa acuminata. In this research, the ethanolic leaf extracts of the selected plant sample was tested for the following secondary metabolites: alkaloids, flavonoids, tannins, saponins, glycosides and terpenes. The antibacterial assay of the fractionated leaf extract, using three different solvents with their increasing polarity, namely ethyl acetate, acetone and methanol was tested against a gram-positive bacterium (Staphylococcus aureus) a gram-negative bacterium (Escherichia coli). The crude ethanolic extract showed a positive results to all of the aforementioned phytochemical tests. However, the fractionated leaf extracts of ethyl acetate, acetone and methanol were unable to inhibit the growth of the tested microbial samples. This is in agreement with the results of the related species reported in the literature wherein there is no inhibition in the bacterial growth.

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