Antiangiogenic properties of Averrhoa bilimbi Linn, and Syzygium polycephaloides using chick chorioallantoic membrane assay

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Averrhoa.

Abstract

The process of angiogenesis has a major contribution to the development of tumor which supplies the tumor several growth factors such as vascular endothelial growth factor (VEGF) and Angiopoietin-1 and 2. In this study, the antiangiogenic properties of Averrhoa bilimbi Linn. and Syzgium polycephaloides were studied using chick chorioallantoic membrane (CAM) assay. Averrhoa bilimbi Linn. methanolic leaf extract was subjected to partial fractionation using column chromatography. The collected ten fractions were tested for their antiangiogenic activity using CAM assay. Only fractions 1 and 10 gave a significant antiangiogenic activity. The two fractions were partially characterized using gas chromatography-mass spectrometry (GC-MS). The gas chromatogram showed 16 and 9 major peaks for fractions 1 and 10 respectively. The major components found in the aforementioned fractions are n-hexadecenoic acid, phytol, vitamin E, oleic acid, choleste-5-en-3-one, (2R, 3S)-2, 3-Dimethylmalate and cis-9, 12, 15-octadecatrienoic acid methyl ester.

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