Anti-angiogenic activity of selected roasted coffee bean extracts using chick chorioallantoic membrane assay

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Bachelor of Science in Biochemistry




Angiogenesis, the formation of new blood cells, is essential for tumor growth, progression and metastasis. Epidemiological studies have shown that coffee consumption is associated with a low incidence of cancer. The coffee beans of C. arabica, C. liberica, C. excelsa and C. robusta were roasted were extracted using 80% ethanol as the solvent. To determine their anti-angiogenic activity, the extracts were subjected to chick chorioallantoic membrane assay with a concentration of 3 μg/mL, 30 μg/mL and 100 μg/mL. Among the four coffee species, C. liberica exhibited the highest anti-angiogenic activity with a percent inhibition of 67.31% at 3 μg/mL. Light roasted coffee samples presented the highest anti-angiogenic activity when compared to medium and dark roasted coffee samples, regardless of the concentration.

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