Title

Green synthesis of silver nanoparticles from Annona muricata leaves extract against Staphylococcus aureus and escherichia coli

Date of Completion

7-2019

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Annona, Metal Nanoparticles, Staphylococcus aureus, Escherichia coli

Abstract

Silver nanoparticles have been throughly researched for different kinds of uses, such as its antibacterial activities. The green synthesis of silver nanoparticles is oftenj done with different methods. The Annona muricata leaf extract is used to synthesize silver nanoparticles, and it had been tested against Staphylococcus aureus and Escherichia coli. Different concentrations of silver nitrate and extract are incubated with varying amount of time. the solutions are checked for the formation and stability of silver nanoparticles using a UV-Vis spectrophotometer, the solution having the highest peak value is considered the optimal condition. The optimal solution for the non-centrifuged agNP solutions was found using 7mL of the extract with a peak of 388 nm. When diluted by 1:3, the peack is at 430 nm, with an absorbance value of 0.094. it is then subjected to FTIR spectroscopy, SEM, EDX, and antibacterial testing. The SEM and EDX results showed that the AgNPs are spherical and clustered shaped, with an average size of 62.2 nm, and with purity 48.72%. The antibacterial testing results revealed that the non-centrifuged AgNP solution is effective against Staphylococcus aureus while the centrifuged AgNP solution, consisting of centrifuged silver nanoparticles diluted with distilled water, is effective against Eschrichia coli.

First Advisor

Eduardo Tibayan Jr.

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