Comparison of the anti-angiogenic effect of ethanolic extract of Garcinia mangostana (mangosteen) pericarp against trastuzumab using chorioallantoic membrane (CAM): an experimental study

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Clinical Pharmacology


Garcinia mangostana, Ethanol, Trastuzumab, Chorioallantoic Membrane


The objective of this study was to compare the anti-angiogenic activity of 3 different concentrations of ethanol extract of Garcinia mangostana (mangosteen) pericarp with Trastuzumab and with distilled water on 10-day-old Ana luzonica (Philippine duck) eggs. Ten-day-old-duck eggs were treated with three (3) different concentrations of mangosteen (10ug/ml, 50ug/ml, 100ug/ml), Trastuzumab (100ppm), and distilled water. After an incubation period of 48 hours, the number of collaterals of chorioallantoic membrane in different treatment groups were compared and analyzed with a level of significance of 0.05 and a confidence interval of 95% via one-way-ANOVA followed by Tukey Test. Results revealed that all concentrations of mangosteen suppressed angiogenesis in a dose dependent manner, although insignificantly when compared to the negative control (distilled water). However, mangosteen extract with 50 ug/ml and 100 ug/ml concentrations were comparable to Trastuzumab, which can be attributed to α-Mangosteen. Therefore, with the correct dosage, Garcinia mangostana pericarp extract can be an alternative to standard anti-angiogenic medications.

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