Anti-bacterial activity and potential anti-quorum sensing activity of crude extracts of piper nigrum corns against Staphylococcus aureus and Escherichia coli

Date of Completion

2016

Document Type

Thesis

Degree Name

Clinical Pharmacology

Keywords

Piper nigrum, Staphylococcus aureus, Escherichia coli, Anti-Bacterial Agents

Abstract

Antibacterial property of water and methanol extact of black pepper corns against staphylococcus aureus and escherichia coli was tested in this study. Antibacterial property was tested via disk diffusion assay. It was done on 3 replicates. Anti-quorum sensing activity was also done after the antibacterial assay. Staphylococcus aureus were grown in blood agar with the extracts mixed with it. Inhibition of hemolysis would mean inhibition of quorum sensing by the extracts. No extract possessed antibacterial property and only the water extract of black pepper corns showed anti-quorum sensing activity in all replicates. Methanol extract did not show any antiquorum sensing activity. The results suggested that the molecules responsible for the anti-quorum sensing activity was polar.

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