Photodynamic activity of anthocyanin from Basella alba linn (Alugbati) berries against Staphylococcus aureus

Date of Completion

6-2019

Document Type

Research Project

Degree Name

Grade 12

Keywords

Photodynamic activity, anthocyanin, Photochemotherapy, Anthocyanins, Basella alba Linn, Staphylococcus aureus

Abstract

The emergence of bacteria that are multi-drug resistant can pose a great threat in the health of the public. This decreases the ability of antibiotics to fight against infections. Among these antibiotic-resistant bacteria is Staphylococcus aureus which can both be commensal and pathogenic. Some of its harmful effects include skin, bloodstream, bones and joint infections. Due to this problem, the researchers explored on the field of photodynamic therapy (APDT). This is a non-invasive method which uses light to activate a photosensitizer which changes from a ground state to an excited singlet state that can create a reaction to damage microbial cells. In this study, the researchers have used anthocyanin extract from alugbati berries enhanced with copper chloride as photosensitizer. It was diluted with 1% dimethyl sulfoxide (DMSO) to achieve the desired concentrations: 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, and 200mg/ml. Its antibacterial effect was tested using Kirby Bauer Disk Diffusion Susceptibility Test with PDT. The agar plates for the experimental group were divided into five sections containing the concentrations. On the other hand, the control group was composed of 1% DMSO for the negative while sulfamethoxazole was used for the positive. The plates were subjected to visible light irradiation for various hours (0 hr, 1 hr and 2 hrs). After the 24-hour incubation, results have shown no zones of inhibition for the different setups. Therefore, there is no significant difference in the zone of inhibition of Staphylococcus aureus applied with various concentrations of the extract.

First Advisor

Carmelette C. Davila

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