Date of Completion

2025

Document Type

Research Project

Degree Name

Grade 12

Keywords

Coffee Grounds, Robusta Coffee, Crude Ethanolic Extract

Abstract

Spent coffee grounds (SCG) are the solid residues generated during coffee preparation, comprising numerous bioactive compounds. With global annual production estimated at 60 million tons, SCGs pose significant waste management challenges, necessitating innovative applications to reduce environmental impact. This study investigated the antibacterial activity of crude ethanolic extract (C.E.E.) derived from SCGs of Coffea canephora against Pseudomonas aeruginosa, a prevalent pathogen in healthcare settings linked to serious infections. The antibacterial efficacy of the C.E.E. was assessed using the Kirby-Bauer disk diffusion method, and its performance was compared against ciprofloxacin, a standard antibiotic. The results revealed that the C.E.E. exhibited a significantly smaller zone of inhibition (8.333 mm) compared to ciprofloxacin (42.667 mm), indicating markedly lower antibacterial efficacy. This significant difference, supported by statistical analysis (p < 0.001), underscores the potential limitations of SCG extracts in combating bacterial pathogens. These findings highlight the need for further investigation into optimizing extraction methods, enhancing the antibacterial properties of SCGs, and exploring their eco-friendly applications in sustainable practices.

First Advisor

Pauline E. Cueno, LPT, MST

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