Date of Completion


Document Type

Research Project

Degree Name

Grade 12


Juncus bufonius, Frog Grass, Escherichia coli, Antimicrobial Agents


This study mainly focuses on Juncus bufonius (Frog Grass) being used as an antimicrobial agent against Escherichia Coli. The researchers tested it with its antimicrobial activity, capabilities, and effectiveness against the said bacteria. It went under the method Agar Disc Diffusion Protocol (USP 30 - NF 25), which gave the researchers the capacity to gather significant data that purely benefits our research on determining the effectiveness against Escherichia Coli (E. Coli). Testing the effectiveness of Juncus bufonius will be against this said bacteria to determine its Aseptic properties. Conducting an In Vitro study allowed the researchers to isolate specific cells, bacteria, and viruses and study each single one of them without having to look at the whole organism. That was conducted in Department of Science and Technology (DOST) located in Taguig, Manila. Measuring the diameters of every Extract that was applied in the Agar Plates individually. This will overall help us analyze and determine the exact zone of inhibition. In result, we used an ethanol solution for extracting the frog grass. The frog grass extract ranging from 100%,75% and 25% of ethanol. Findings shown that the frog grass in 100% and 75% has a mild effect against the Escherichia coli, but the 25% of extract had no reactivity against the bacteria.

First Advisor

Mark Lawrence F. Fabregas