Date of Completion

7-2019

Document Type

Thesis

Degree Name

Grade 12

Keywords

Plant Extract, Escherichia coli, Staphylococcus aureus, Antibacterial agents, antibacterial properties, oregano, tawa-tawa, Ipomoea batatas, Origanum vulgare, Psorolea corylifolia, sweet potato

Abstract

In this study, the leaf extracts of Ipomoea batatas, Origanum vulgare, and Psorolea corylifolia were tested for antibacterial properties against Escherichia coli and Staphylococcus aureus using disc-diffusion assay. Ethanol was used as a solvent while Mueller Hinton agar plates were used to test for the antibacterial action of the plant extracts. The result of the zones of inhibition showed that the leaf extract of Ipomoea batatas, Origanum vulgare, and Psorolea corylifolia exhibit no significant effect against the two sample bacteria. For the difference in relation to their different concentrations, Origanum vulgare lack any zone of inhibition and Ipomoea batatas showed an insignificant difference (P value > 0.05) on both bacteria. Psorolea corylifolia's zone of inhibition showed no significant difference against Escherichia coli; however, it showed a significant difference (P value = 0.00) when tested against Staphylococcus aureus. The different concentrations of Ipomoea batatas, Origanum vulgare, and Psorolea corylifolia leaf extracts did not yield a >15mm zone of inhibition. This means that the plants did not meet the standard zone of inhibition.

First Advisor

Pancho E. Ercilla

Available for download on Saturday, May 15, 2021

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