Title

Detection of cryptosporidium parvum and analysis of the sanitary practices in the maintenance of selected recreational pools in Bacoor and Dasmarinas City, Cavite

Date of Completion

7-2019

Document Type

Capstone Project

Degree Name

Grade 12

Keywords

Cryptosporidium parvum, parasite, Parasites, swimming pools, chlorine, sanitary practices

Abstract

Protocols adapted from Masangkay, et. al (2016) and Paller, et. al (2017) were utilized to effectively process the samples. Modified Ziehl-Neelsen staining was performed on the sediment samples. After which, microscopic analysis was executed to determine the presence of Cryptosporidium parvum from the samples. The pools were also subjected to profiling through questionnaire format, adopted from the survey by the CDC (2006). Out of six samples, all are negative from Cryptosporidium parvum oocysts. This is mainly due to the different sanitary practices in the maintenance of the establishments, which are qualified in the standards according to Sanitation Code of the Philippines (P.D. 856), and the pH and chlorine levels further confirmed through water analysis that affected the presence of the said parasite.

First Advisor

Dennis Yu Perona

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