Date of Completion


Document Type

Research Project

Degree Name

Grade 12


Antimicrobial, Antibacterial agents, ocimum sanctum, escherichia coli, staphylococcus aureus


This study utilizes a True Experimental Research Design and was presented using a post-test control only design. Erlenmeyer Flask compo0nents of Tulsi or holy basil, distilled water was applied as solven for the extraction while dried powdered leaves was service as the solute. Furthermore, ethanol was evaporated using a rotary evaporator and crude extract was transferred and stored into a vials. The extraction was continued when the solution on the apparatus became cleaer. Kirby-Bauer method (Disc Diffusion Method) was also used for the evaluation of the antimicrobial capabilities of the Ocinum sanctum leaf crude extract. As a result of the experiment, no zones of inhibition was dound on all ten plates, both on E. coli adn S. aureus due to the development of antibiotic resistance on both bacteria. With these results, the researchers concluded that the crude leaf extract of O. sanctum is ineffective against Ampicillin resistant E. coli and S. aureus.

First Advisor

Vivian B. Ramirez