C-Reactive Protein–based Screening of People with Tuberculosis Symptoms: A Diagnostic Accuracy Study

Publication Date

3-1-2025

Document Type

Article

Publication Title

American Journal of Respiratory and Critical Care Medicine

Abstract

Rationale: C-reactive protein (CRP)-based tuberculosis (TB) screening is recommended for people with HIV. However, its performance among people without HIV and in diverse settings is unknown. Objectives: In a multicountry study, we aimed to determine whether CRP meets the minimum accuracy targets (sensitivity > 90%, specificity > 70%) for an effective TB screening test. Methods: Consecutive outpatient adults with cough >2 weeks from five TB endemic countries in Africa and Asia had baseline blood collected for point-of-care CRP testing and HIV and diabetes screening. Sputum samples were collected for Xpert MTB/RIF Ultra (Xpert) testing and culture. CRP sensitivity and specificity (5 mg/L cut-point) was determined in reference to sputum test results and compared by country, sex, and HIV and diabetes status. Variables affecting CRP performance were identified using a multivariate receiver operating curve regression model. Measurements and Main Results: Among 2,904 participants, of whom 613 (21%) had microbiologically confirmed TB, CRP sensitivity was 84% (95% confidence interval [CI], 81–87%) and specificity was 61% (95% CI, 59–63%). CRP accuracy varied geographically, with higher sensitivity in African countries (>91%) than Asian countries (64–82%). Sensitivity was higher among men than women (86% vs. 78%; difference, 18%; 95% CI, 1–15%) and specificity was lower among people with HIV than people without HIV (64% vs. 45%; difference, 119%; 95% CI, 13–25%). Receiver operating curve regression identified country and measures of TB disease severity as predictors of CRP performance. Conclusions: Overall, CRP did not achieve the minimum accuracy targets, and its performance varied by setting and in some subgroups, likely reflecting population differences in mycobacterial load.

First Page

499

Last Page

506

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