Date of Completion

2023

Document Type

Thesis

Degree Name

Bachelor of Science in Pharmacy

Keywords

Antibacterial, Katmon

Abstract

Antimicrobial resistance is a global issue caused by the misuse of antibiotics, highlighting the demand for new ways to combat bacterial infections. Bacterial biofilms are major contributors to antimicrobial resistance, necessitating the development of antibiofilm agents. As of today, the U.S. Food and Drug Administration has not yet approved any antibiofilm agent (Yu et al., 2019). In this study, a bioassay guided fractionation of Dillenia philippinensis Rolfe (Katmon) leaf extract was performed to assess its antibacterial and antibiofilm activity against Staphylococcus aureus and Pseudomonas aeruginosa. The Katmon leaves that were collected were garbled, shade dried and milled before subjecting to serial exhaustive extraction using solvents of increasing polarities: dichloromethane, ethyl acetate, and methanol. The extractives were then subjected to minimum inhibitory concentration (MIC) determination prior to antibiofilm assays. It was found out that among the extractives, KDCM exhibited highest antibacterial activity against S. aureus. The ability to inhibit biofilm formation and eradicate pre-formed biofilm were assessed in the study. The results showed KMeOH having the highest inhibitory activity while KDCM showed highest eradication activity, both against S. aureus. The study decided to further fractionate KMeOH through column chromatography and the fractions were pooled using thin-layer chromatography. The study was able to obtain seven Katmon pooled fractions (KPF1, KPF2, KPF3, KPF4, KPF5, KPF6, and KPF7) which were again tested for its antibacterial and antibiofilm activity. Only KPF1 showed antibacterial activity against both S. aureus and P. aeruginosa. KPF1 and KPF2, on the other hand, showed biofilm formation inhibition against S. aureus and KPF7 against P. aeruginosa. No pooled fractions, however, showed an activity in eradicating the pre-formed biofilm of both bacteria.

First Advisor

Jhulez Anthony B. Dayrit

Second Advisor

Renz Angelo G. Bunquin

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