Determination of the anti-angiogenic activity of the practions of the ethyl acetate extract of Moringa oleifera lam. leaves using CAM assay

Date of Completion

5-2019

Document Type

Thesis

Degree Name

Bachelor of Science in Pharmacy

Keywords

Moringa oleifera, Angiogenesis Inhibitors, CAM assay, anti-angiogenic activity

Abstract

The ethyl acetate crude extract of Moringa oleifera leaves was prepared by exhaustive soxhlet extraction and was fractionated by column chromatography using hexane, ethyl, acetate, and methanol as solvents. The fractions were screened by performing phytochemical test and analyzed by conducting thin-layer chromatography. The anti-angiogenic activity of the fractions was tested using in-vivo chorioallantoic membrane (CAM) assay. The results were evaluated using a scoring system and analyzed by non-parametric statistical tests. The results of phytochemical analysis showed that only methanol extract tested positive in the presence of carbohydrates and alkaloids. However, a standard of each test for comparison was not performed which makes the results inconclusive. Results for the TLC analysis confirm that the fractionation process was successful in separating compounds based on their different polarities. Lastly, CAM Assay obtained results that methanol fraction extract showed the most potent-antigiogenic activity among the other extracts.

First Advisor

Timothy Jay L. Bengala

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