Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Pharmacy

Keywords

Herbal Medicine, FTIR, Chemometrics, Lagundi (Vitex negundo L.), Environment, Environmental condition, TLC, Maceration, Soil Analysis, NPK

Abstract

This study investigates the influence of environmental conditions, including soil pH, temperature, and geographical variations, on the chemical composition of Lagundi (Vitex negundo L.) leaves collected from selected areas in CALABARZON, Philippines. Fourier Transform Infrared (FTIR) Spectroscopy was utilized to analyze and compare the spectral fingerprints of Lagundi samples obtained from different locations with a standard reference sample from Herbanext. The analysis focused on identifying significant wavenumbers corresponding to key functional groups present in Lagundi leaves, which are indicative of their bioactive compounds. To further interpret the spectral data, multivariate statistical techniques such as Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were employed to assess the correlation between environmental factors and variations in chemical composition. The findings revealed distinct spectral similarities among Lagundi samples across different areas of CALABARZON, suggesting that despite geographical and environmental differences, the overall chemical profile remains relatively consistent. This study highlights the importance of understanding environmental influences on medicinal plant composition, which is crucial for ensuring the quality, efficacy, and safety of herbal medicines like Lagundi. The results provide valuable insights for the standardization of raw materials used in pharmaceutical applications, supporting the development of quality control measures in the production of herbal formulations. Additionally, the use of FTIR Spectroscopy as a rapid and non-destructive analytical tool demonstrates its potential in the routine assessment of herbal products, ensuring their reliability for medicinal use.

First Advisor

Darwin Japheth L. Eusebio, RPh

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