Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Pharmacy

Keywords

Albumin, Chromatography, Drug-Herb Interactions

Abstract

Drug-herb interactions from the concomitant use of conventional drugs and herbal medicine either hinder the therapeutic activity or increase the toxicity of drugs, posing a public health concern. Zonal elution chromatography was established to detect solute binding to serum proteins such as albumin, with methods adapted from chromatographic displacement immunoassay. With corticosteroids widely prescribed for inflammatory diseases, alongside herbal medicine such as garlic, this study aims to establish weak affinity chromatography as a method to detect distribution-mediated drug-herb interactions between prednisone, hydrocortisone, and cortisone with garlic’s anti- inflammatory constituent, allicin. Individual sample injections and paired injections of steroid and allicin samples were introduced into a prepared column, containing 1.815 mM of immobilized human serum albumin, and ultraviolet spectroscopy was used to detect the changes in retention time and area under the curve. A higher area under the curve values was observed when allicin and corticosteroids were injected individually into the human serum albumin column. The area under the curve values significantly decreased when allicin and a corticosteroid were combined with one another. Among the different methods performed, the displacement method showed a decrease in allicin’s retention time. Weak affinity chromatography has identified that allicin has an interaction with the steroids analyzed. This is supported by the changes in the retention time, dissociation constant, and area under the curve of allicin. The results show the utility of HSA-WAC as a routine analytical method to assess the safety of herbal medicines. This can be an alternative to more costly procedures, in terms of protein usage, such as equilibrium dialysis, ultrafiltration, and ultracentrifugation.

First Advisor

Timothy Jay L. Bengala, RPh, MSPh

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