Title

Isolation of bacteriophage specfic to pseudomonas aeruginosa BIOTECH 1335 strain from raw sewage water of local sewage treatment plant in Cavite

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Pseudomonas aeruginosa, Bacteria, Sewage

Abstract

This research employed a descriptive design wherein it utilized a systematic and scientific approach in collecting raw sewage water samples and tested them with the employed methods to observe the formation of bacteriophage which is focused on gathering numerical data and using it to explain a reliable result. The results of the study showed that the double layer agar technique shows that the formation of plaques indicates a presence of bacteriophage and the statistic shows that the mean number of plaques in the sewage group is significantly higher than that in the control group with a data of t=10.499, 95% Cl 16.95, 24.87; p<0.001. The study concluded that the isolated bacteriophage from raw sewage water shows that there is an abundant presence of Pseudomonas aeruginosa BIOTECH 1335 strain phage. Phage inhibits bacterial biofilm by lysing the host's bacteria and further infecting other bacteria. Since the presence of plaques, which are composed of lysed Pseudomonas aeruginosa bacteria, basically indicate the present of bacteriophages.

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