In-vitro assessment of the antiplasmodial effect of Blumea balsamifera (sambong) stem and roots against Plasmodium falciparum
Date of Completion
2018
Document Type
Thesis
Degree Name
Bachelor of Science in Medical Laboratory Science
Keywords
Plasmodium falciparum, Plasma
Abstract
Experimental method was used in this study. The study performed different procedures that include materials, reagents and equipment. Ninety five percent (95%) ethanol was utilized for the suspension of the macerated plant samples. Phosphate buffered saline was used to dilute the extracts in their respective concentrations, 37% hydrocholoric acid was used for dilution of sodium doceyl sulfate in the assay as the surfactant, these reagents were bought from the Center for Basic Biomedical Research, Angelo King Bldg., De La Salle University Medical Center, Dasmarinas, Cavite. During the preparation of the plant extract, the researchers used the Rotary Evaporator provided by the said facility. The researchers also used a lyophilizer from the College of Pharmacy in De La Salle Medical and Health Sciences Institute, Dasmarinas, Cavite. This allowed the researchers to complete the extract that was used against the Plasmodiumfalciparum. Based on the results, it showed that all of the plant extract concentrations has an absorbance of below negative control (<1.685). Researchers concluded that the results are insignificant and Sambong plant extracts may display antiplasmodial activity against Plasmodium falciparum.
APA Citation
Aflleje, I. K., Cuevas, M. L. B., Maglabe, M. G., Papa, C. B., & Reyel, K. (2018). In-vitro assessment of the antiplasmodial effect of Blumea balsamifera (sambong) stem and roots against Plasmodium falciparum. [Bachelor's thesis, De La Salle Medical and Health Sciences Institute]. GreenPrints. https://greenprints.dlshsi.edu.ph/bsmls/40/