Title

Comparison of point of care and clinical laboratory analysis of cystatin-C levels in patients with renal nephropathy

Date of Completion

2017

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Cystatin C, Point-of-Care Testing, Clinical Laboratory Techniques

Abstract

This study utilized the descriptive-comparative research design. Two methods were applied, point of care test and clinical laboratory testing, both employing the Latex-particle Enhanced Immunoturbidimetric Assays (PETIA) principle to be able to measure and analyze the levels of Cystatin-C for each of the participants of the study in order to rectify whether POCT results were comparable with the standard laboratory results. Purposive sampling was used as a sampling technique. The preferrred sample size was a total of 32 patients in De La Salle University Medical Center (DLSUMC) following the important inclusion criteria of those with renal disease (nephropathy) either undergoing dialysis or not, male or female with ages ranging from 18 to 65 years old to determine the Cystatin C level results in POCT and standardized clinical laboratory. Exclusion cirteria include those respondents that had taken corticosteroids, and those characteristics that disqualify prospective subjects from inclusion in the study. The sample collection were taken from each patients. One sample was tested for Cystatin-C POCT using capillary blood and the other sample for teh actual Cystatin-C laboratory analysis using venous blood. Samples from capillary blood were collected by a registered medical technologist while the venous blood was collected from the IV line of the dialysis patients by their assigned nurse. The samples were sent immediately and without delay for the Cystatin-C analysis in the National Kidney and Transplant Institute (NKTI). Data was recorded as the laboratory results were released (standardized clinical laboratory testing). For the results in Cystatin-C POCT, an immediate reading within 10 minutes was made. Results were recorded on the original data collection sheet and were secured for data analysis. Data was analyzed through mean, standard deviation, t-test and f-score. The study concluded that the results of the POCT used in determining Cystatin-C levels were proven to be unvarying and strongly comparable to the results of clinical laboratory test. Therefore, point of care testing using capillary blood sample, which can deliver immediate result an at approximately 10 minutes, can be excellent alternative method in detecting Cystatin-C levels especially in emergency cases.

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