Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Antibacterial Activity

Abstract

The researchers aim to determine the bioactive compounds of the crude rambutan leaves extract, identify the presence of silver nanoparticles using Ultraviolet-Visible (UV-Vis) Spectroscopy, Fourier Transform Infrared (FTIR) Spectroscopy and Scanning Electron Microscope (SEM) and Energy Dispersive X-ray Spectroscopy (EDX), and to determine the Minimum Inhibitory Concentration (MIC) of green synthesized silver nanoparticles from Rambutan leaves extract against Pseudomonas aeruginosa, an aerobic gram-negative bacterium which can cause nosocomial infections associated with high morbidity and mortality rates in immunocompromised patients. The synthesized rambutan leaves extract was characterized using UV-Vis Spectroscopy, FTIR Spectroscopy and SEM. The results from UV-Vis Spectroscopy showed peaks for the synthesized silver nanoparticles with absorbance values of 0.071, 0.045, 0.044, and 0.043 at wavelengths of 340, 436, 446, and 460 nm, respectively. Meanwhile, FTIR Spectroscopy showed peaks of 3,400; 3,200; 2,260, 1,680; and 1,350 cm−1 as it is associated with carboxyl (-C=O) and (-NH) amine groups which acts as a reducing and stabilizing agent in AgNPs synthesis. SEM images showed agglomerated rod-shaped and spherical structures of the silver nanoparticles. The results obtained from Wells A, B, & C shows that the green synthesized silver nanoparticles of the rambutan leaves extract have potential antibacterial activity against P. aeruginosa as Wells A & B at a concentration of 0.5 mg/mL and Well C at 1 mg/mL.

First Advisor

Carolina Capili, RMT

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