Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Vigna unguiculata (Sitaw), Phaseolus lunatus (Patani)

Abstract

Lectins are carbohydrate-binding proteins found in various organisms, such as plants and bacteria and has the ability to cause agglutination of cells. This study explores the lectin-like activity isolated from the extracts of Vigna unguiculata (sitaw) and Phaseolus lunatus (patani) seeds against Escherichia coli and Enterococcus faecalis through evaluation of agglutination by absorbance reading and microscopy. Results demonstrated that both plant extracts agglutinated E. coli at all tested concentrations, with a significant decrease in absorbance (p < 0.05) indicating substantial aggregation. The decrease in absorbance for E. faecalis was not statistically significant (p > 0.05). Independent samples t-tests revealed no significant difference in the effectiveness of V. unguiculata and P. lunatus extracts in agglutinating either E. coli or E. faecalis across the tested concentrations. These findings highlight the potential of both V. unguiculata and P. lunatus extracts as rapid identification methods due to their consistent and comparable agglutinating activity against the tested bacteria.

First Advisor

Theo John M. Segismundo, RMT

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