Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Methicillin-Resistant Staphylococcus aureus, Dayap Peels, Pectin, Kirby-Bauer Disk Diffusion Assay, Minimum Inhibitory Concentration

Abstract

Methicillin-Resistant Staphylococcus aureus (MRSA) is a bacterium that is known to be resistant to several antibiotics. This has developed resistance to numerous antibiotics which makes it difficult to treat. Pectin extract obtained from Citrus × aurantiifolia (Dayap) peels was assessed for its antimicrobial activity against MRSA. Through a series of laboratory experiments and analyses, this study explored the efficacy of pectin as a natural antimicrobial agent, aiming to contribute to the development of alternative treatments for MRSA infections that are organic and cheap to produce. The study design encompasses extraction procedures, identification procedures, antimicrobial assays, and data analysis techniques to assess the inhibitory effects of pectin on MRSA growth. Based on the results, pectin showed no inhibitory effects to MRSA Bionumber (BN): 05040207376327 growth using Kirby-Bauer disk diffusion assay. Moreover, this study performed a broth dilution method to determine the minimum inhibitory concentration (MIC) using 5, 2.5, 1.25, 0.625, 0.312, and 0.156 mg/mL concentrations and none of these were successful in inhibiting MRSA growth.

First Advisor

Christine Ann Aromin, RMT, DTA, MPH(c)

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