Date of Completion

2023

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Post-cleaning, cleaning evaluation, swabbing, cleaning practices, spectrophotometry, absorbance, optical density, plate count

Abstract

According to the Cambridge Dictionary (n.d.), the bathroom is defined as a space that is constantly used to relax, groom, and cleanse aside from its main function, which is for urination and defecation, thus the need for this location to be constantly sanitized. With the constant use of the comfort rooms, the transmission of bacterial through different surfaces in the comfort room is likely. Through swabbing selected surfaces in the comfort rooms, namely; flush levers, bidet handles, and stall locks, the efficacy of cleaning is evaluated. With statistical analysis, the microbial concentration, significant difference of cleaning practices, as well as the involvement of time and the three sites of swabbing were assessed. A total of 535 samples were collected and 146 were turbid after 24 hours and 48 hours of incubation. After incubation, samples were plated and incubated for 24 hours. After 24 hours of incubation, colonies were counted to determine the microbial concentration on the different surfaces. Succeeding the colony counting, results have shown that the stall locks had the highest mean of microbial concentration among all of the highest surfaces, and the time with the highest microbial concentration is during the midday. In conclusion, the initial microbiological load collected from the sites prior to immersion in BHIB is all within clean surface requirements.

First Advisor

Maria Katrina B. Pilit

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