Date of Completion

2023

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Aloe, Salmonella, ciprofloxacin, MIC, MBC, Antibacterial

Abstract

Millions of people worldwide acquire food borne diseases annually and among its leading cause is Salmonella typhimurium, a serovar of gram-negative bacteria Salmonella enterica. Generally, it is susceptible to antibiotics such as fluoroquinolones but there has been emergence of antimicrobial resistant strains due to antibiotic misuse and overuse. This leads to the development of alternative source of antimicrobial treatment such as plants. This study focuses on evaluating the antibacterial property of Aloe vera ethanolic leaf extract against Salmonella Typhimurium using Kirby-bauer susceptibility testing, MIC and MBC assay. The results showed that leaf extract with a concentration of 2 mg/mL, 1 mg/mL, 0.5 mg/mL and 0.25 mg/mL yielded a zone of inhibition of 29.5 mm, 25.4 mm, 17.8 mm and 10.9 mm, respectively. On the other hand, 0.125 mg/mL, 0.06 mg/mL, 0.03 mg/mL, and 0.016 mg/mL concentrations did not exhibit any zone of inhibition. The MIC assay showed that the 0.25 mg/mL is the last concentration that were able to inhibit at least 50% of Salmonella Typhimurium and the MBC of the Aloe vera extract is at 2 mg/mL. In comparison to the positive control, ciprofloxacin yielded 48.1 mm zone of inhibition, an MIC of 0.016 mg/mL and an MBC of 0.25 mg/mL, suggesting a significant difference between the Aloe vera extract.

First Advisor

Clarence Witty H. Mendoza-Fenol

Download

Share

COinS