Title

Correlation between the levels of cotinine with serum lysozyme among cigarette smokers in Brgy. Punta II, Tanza, Cavite

Date of Completion

2017

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Smoking, Cotinine, Muramidase

Abstract

This study used descriptive-correlational research design. Using convenience type of sampling technique, 80 participants were chosen. Specimen collection was done at Brgy. Punta II, Tanza, Cavite, Philippines. Data collection, on the other hand, was performed in the Centro Escolar University Manila. The study used two Cotinine ELISA kits and two Lysozyme (ELISA kits. Ninety-six wells were prepared with 40 samples and 16 standards done in duplicates. Cotinine and lysozyme standard solution were prepared in concentrations of 80, 40, 20, 10, 5, 2.5, 1.25 and 0.628 and 40, 20, 10, 5, 2.5, 1.25, 0.625 and 0 respectively. A 100uL washed standard, blank or sample was added with biotinylated detection Ab, HRP conjugate, substrate and stop solution. Each addition of reagents have an interval of washing> Optical density were measured using microplate reader calibrated at 450nm. One-Way ANOVA results showed that the greatest mean concentration of serum cotinine and lysozyme is 0.58 ng/mL obtained from moderate smoker and 15.29 ng/mL obtained from non-smoker, respectively. Least mean concentration of serum cotinine and lysozyme is 0.34 ng/mL obtained from light smoker and 3.30 ng/mL obtained from moderate smoke, respectively. Results showed that there is an inverse relationship between serum cotinine and serum lysozyme. The study concluded that the consumption of tobacco has a significant inverse relationship of serum cotinine, a derivative of nicotine, in serum lysozyme. Therefore, smokers have increased risk of health complications due to decreased in lysozyme.

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