Date of Completion

2025

Document Type

Thesis

Degree Name

Bachelor of Science in Life and Health Sciences

Keywords

Leguminosae, Antibacterial, Antimicrobial, Soybean, Glycine max, Pigeon pea, Cajanus cajan, Mung bean, Vigna radiata, Lima bean, Phaseolus lunatus

Abstract

Faced with the growing threat of antibiotic-resistant bacteria, this study explored whether everyday legumes—soybean (Glycine max), mung bean (Vigna radiata), pigeon pea (Cajanus cajan), and lima bean (Phaseolus lunatus)—could serve as natural sources of antibacterial proteins. Crude proteins were extracted from the seeds and confirmed using the Biuret test. Their antibacterial potential was then tested against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa through the disk diffusion method. Despite careful procedures and repeated trials, the extracts showed no observable zones of inhibition. Despite negative results the study offered valuable insights: crude extracts alone may not be potent enough; active compounds might require further purification or concentration; and factors like environmental conditions or extraction techniques could affect results.

Although no antibacterial activity was detected, the study still adds to the broader effort to discover plant-based antimicrobial agents. More importantly, it highlights the importance of persistence, critical analysis, and refining methods in scientific research—reminding us that even negative results can guide the next steps toward meaningful discoveries.

First Advisor

Illuminada A. Ronio

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