Date of Completion


Document Type


Degree Name

Bachelor of Science in Biochemistry


Prebiotics, beta-Galactosidase, Bifidobacterium bifidum, Plants


A wide variety of secondary metabolites are present as natural products. Most of them possess various pharmacological properties such as prebiotic activity. Secondary metabolites including alkaloids, phenolic acids, and terpenes, and prebiotics such as inulin and Fructooligosaccharide were examined to know their prebiotic activity. To determine the possible inhibitory effect of prebiotics and secondary metabolites on the beta-gal of the Bifidobacterium bifidum. Parameters were checked which included the binding affinity and bond distances in Angstrom. Using the Autodock Vina program in UCSF Chimera Software, the Beta-Galactosidase complex docking was carried out. Results showed that Rutin had the highest binding affinity among 29 metabolites with -9.7 kcal/mol and Inulin had the shortest distance with 2.74 Å and 3.87 Å towards Glu 161 and Glu 320 respectively, and thus were considered as potential inhibitors of Lactose. Nerol and Citral had the lowest binding energy with a binding affinity of -4.9 kcal/mol.

First Advisor

Walter Aljohn T. Espiritu