Title

Isolation, purification and characterization of Medinilla magnifica leaf extracts

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Plants, Medicinal

Abstract

Medinilla magnifica is an ornamental plant endemic to the Philippines belonging to Melastomatica family. In this study, phytochemical investigation and purification of the three crude extracts of M. magnifica leaves were carried out. The isolated compounds were characterized by chemical and spectroscopic means. In addition, the crude and purified extracts were tested against representative microbes i.e. gram positive bacteria (S. aureus), gram-negative bacteria (E. coli), and fungi (C. albicans). UV-Vis spectrometry displayed peaks between 210 and 240 nm, indicative of terpenes. This is supported by the functional groups observed using FTIR. Antimicrobial assay results showed that strong microbial growth inhibitory activity against S. aureus for the petroleum ether and dichloromethane crude extracts at concentration of 100 mg/ml and 10 mg/ml, respectively. Moderate microbial growth inhibitory activity was observed for the ethanol crude extracts at 100mg/ml.

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