In vitro screening of antimicrobial activities of selected Philippine Clerodendrum and Rotheca species

Date of Completion


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Degree Name

Bachelor of Science in Biochemistry




The crude extracts pf Clerodendrum intermedium, Clerodendrum qudriloculare, and Rotheca incisa from family Lamiaceae in different solvents were screened against one-gram positive (S. aureus) and one gram-negative (E.Coli) bacteria using the agar well diffusion technique and micro both dilution technique. Methanol extracts showed strong antibactrial activity against both bacteria while DCM extracts only worked against S. aureus. In contrast, hexane extracts did not show any antibacterial activity. Potent concentrations ranged from 10 mg/mL to 0.1 mg/mL and the Minimum Inhibitory Concentration for all extracts that showed inhibition was obtained. The methanol and DCM extract with the lowest MIC and most potency against S. aureus is R. incisa (0.125 mg/mL and 2.5 mg/mL, respectively). For the antibacterial activity against E. coli, the methanol extract of C. quadriloculare (2.5 mg/mL) exhibited the lowest MIC. These results show tha possible use of the three selected species in creating a broad range antibacterial formulation.

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