Title

In vitro screening of antibacterial activity of selected merremia species

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Anti-Bacterial Agents.

Abstract

Human infections that are caused by resistant microbes fail to respond to treatment which leads to prolonged illness and premature death throughout the world. Plants are known to have antibiotic properties that are used by the traditional system to relieve various diseases. This study was undertaken to evaluate and compare the antibacterial activity of selected species from the Convovulaceae family, namely: Merremia peltata (linn.) Merr, Merremia vitifolia (Burm.F.) Hallier F., and Merremia Umbellata (linn.) Hallier f., using hexane, dichloromethane and methanol as extracting solvents. Phytochemical analysis showed the presence of phenols, steroid, flavanoids, alkaloid, cardenolides, anthrones and coumarins. The Kirby-Bauer disc diffusion assay was utilized to evaluate the antibacterial potentials of the subject plants. Results revealed that the crude extract of hexane and dcm showed no inhibition in the bacterial strains of S. Aureus and E. coli. Only the crude methanol extract possessed an antibacterial activity in both bacteria at concentrations 10 and 1 mg/mL. Upon further analyses, the minimum inhibitory concentration was determined and was known to be 1.25 mg/mL. It was then concluded that among the three species of Merremia, M. vitifolia has the largest zone of inhibition against the strains of E. coli and S. aureus. Statistical treatment using one-way ANOVA showed no significant difference in the methanol extracts at 10mg/mL against E. coli since the p-value obtained was 0.098, which is greater than 0.05 level of significance. Methanol extracts at 10mg/mL and 1 mg/mL against S. aureus yielded p-values less than 0.05; as well as the extract at 1 mg/mL against E. coli. Thus, this indicates that this plant has potential bioactive substances for medicinal purposes.

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