Title

∝-amylase inhibitory activity of soluble proteins and their peptic hydrolysates from commercially available dried squash seeds (Butong Kalabasa)

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

alpha-Amylases.

Abstract

The general objective of this study was to evaluate the ∝-amylase inhibitory activity of soluble proteins and their peptic hydrolysates obtained from commercially available dried squash seeds (butong kalabasa). Results have shown that the soluble protein concentration for crude extract, crude protein and dialyzed crude protein were 0.905, 0.726 and 0.495 mg/mL, respectively, upon defatting, extraction, ammonium sulphate precipitation and dialysis. Protein profiling via denaturing gel electrophoresis showed prominent protein bands with approximate molecular weights around 49-50, 31, 23, 18 and 10 kDa. In the in-vitro ∝-amylase inhibitory activity asay, peptic hydrolysates obtained from the treatment with higher digestion period (i.e. 4h incubation time) were shown to have higher ∝-amylase inhibitory activities exhibiting 18.25 and 37.83% inhibition of the enzyme for crude extract and dialyzed crude protein, respectively. These results considerable indicated the potential of squash seed proteins and their hydrolysates to help regulate the blood sugar levels by postponing glucose release from starch hydrolysis in vitro.

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