Determination of the hypocholesterolemic effects of citrus peels namely citrus aurantium (dalandan), citrus limon (lemon), and citrofortunella microcarpa (calamansi) on male albino mice (mus musculus)

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Clinical Pharmacology


Citrus, Mice, Hypocholesterolemic


Citrus aurantium (dalandan), citrus limon (lemon), and citrofortunella microcarpa (calamansi) were evaluated for their hypocholesterolemic effects against atorvastatin on male albino mice (mus musculus) by measuring the serum total cholesterol level obtained from the tail of the mice using the tall snip method with a drop of blood placed on a testing strip from a lipid profilekit. Fifteen male albino mice were used, randomly divided into five groups, a negative control group (distilled water), a positive control group and three treatment groups. The serum total cholesterol lowering effects of the citrus peels were compared to commercially available lipid-lowering drug. To know the effects of the peel extracts to the albino mice total cholesterol level, the extracts were gavaged to the mice once a day for two (2) weeks with the concentration of 20ml/kg. Results showed that all treatment groups showed a decrease in the total serum cholesterol levels after two weeks of the induction. However, the decline exhibited by the three citrus peels namely lemon, calamansi, and dalandan were insignificant. Thus, this study was not able to demonstrate the hypocholesterolemic effects of the citrus peels. When compared to the positive (Atorvastatin) and negative (distilled water) control groups, all the treatment groups have shown no significant differences. However, since literature have proven the effectiveness of atorvastatin in lowering blood cholesterol levels, this implication may be deemed as invalid, i.e. atorvastatin has a difference compared with the citrus peels in lowering blood cholesterol levels. Results were attributed to different factors such as improper dosing and its adjustments, small sample size, limited number of replications, and inadequacy of the assessment method.

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