Title

Antimicrobial potential and phytochemical analysis of Mimosa pudica L. (Makahiya) aqueous and methanolic leaf crude extracts

Date of Completion

2017

Document Type

Thesis

Degree Name

Clinical Pharmacology

Keywords

Anti-Infective Agents, Mimosa, Plant Extracts, Phytochemicals, Methanol

Abstract

This research study targeted to determine the phytochemical profile and relate it to the antimicrobial activity of Mimosa pudica L. (Makahiya) aqueous and methanolic leaf crude extracts on Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Salmonella enteritidis. An experimental study consisting of two parts, the phytochemical screening and antimicrobial susceptibility testing involving 6 treatments in triplicates specifically positive control (Ciprofloxacin), negative control (sterile water), aqueous and methanolic leaf crude extracts of Mimosa pudica L. in two (2) different concentrations tested against the aforementioned four (4) bacterial test organisms. The antibacterial property was determined by comparing the zones of inhibition elicited during the Kirby Bauer Susceptibility test. Based on the data gathered, aqueous and methanolic leaf crude extracts of Mimosa pudica L. leaf slightly differed in terms of the phytochemical profile since free fatty acids were exclusively detected in aqueous extract while fats and oil along with steroids and triterpenes were detected only in the methanolic extract. Common constituents detected between the two extracts include tannins, polyphenols, 2-deoxysugar, leukoanthocyanins, gamma-benzopyrone nucleus-flavonoids. Meanwhile, results of antimicrobial susceptibility testing showed no zones of inhibition for all treatments except for the positive control (Ciprofloxacin, 100 mg/ml).

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