Title

Antibacterial activity of camellia sinensis (green tea) leaf extract on the growth rate of staphylococcus aureus, escherichia coli, and salmonella typhi

Date of Completion

2016

Document Type

Thesis

Degree Name

Clinical Pharmacology

Keywords

Green Tea, Plant Extracts, Staphylococcus aureus, Escherichia coli, Salmonella typhi.

Abstract

The antibacterial activity of green tea (camellia sinensis) leaf extract was determined against the growth rate of staphylococcus aureus, escherichia coli, and salmonella typhi. Two methods were done for bacterial susceptibility namely agar gal diffusion and paper disc diffusion. Different concentrations of GT were used such as 625ug, 1250ug, and 2500ug, and these were tested with the controls such as penicillin, ampicillin, and NSS. The green tea extract produced inhibition zone ranging from 0.2-13.4 mm against staphylococcus aureus only. Escherichia coli and Salmonella typhi were not found to be susceptible to the antibacterial activity of green tea. With the susceptibility of staphylococcus aureus to green tea, its effects were compared with the control, and were found to be statistically significant. Moreover, with increasing dose/concentration of the green tea, the zone of inhibition of green tea also increased or became wider. With the two methods used for the antibacterial activity of green tea, it was also found that agar gel diffusion yielded better results than paper disc diffusion method. As per the effects of green tea on the growth rate of the bacteria, no changes were found hours after incubation (zone of inhibition did not changed).

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