Title

The antimicrobial effects of ashitaba (Angelica Keiskei) stem ethanolic extract on staphylococcus aureus, salmonella typhi, and escherichia coli

Date of Completion

2017

Document Type

Thesis

Degree Name

Clinical Pharmacology

Keywords

Anti-Infective Agents, Plant Extracts, Staphylococcus aureus, Salmonella typhi, Escherichia coli, Angelica

Abstract

This study was done to determine the antimicrobial potential of Angelica keiskei stem extract in inhibiting the growth of S. aureus, S. typhi, and E. coli based on the diameters of their zones inhibition. Ten ug of the extract was dried into blank discs and were placed onto culture plates containing the aforementioned bacterial species. Each treatment was done in triplicate with a positive control of ten ug of ampicillin and a negative control of normal saline solution. The plates were observed after 24 hours for S. aureus plates and 16-18 hours for S. Typhi and E. coli. The diameters of the zones of inhibition were measured using a ruler, were inputted in Excel, and analyzed with OpenEpi statistical freeware. Results showed that Angelica keiskei stem extract had insignificantly activity against E. coli and had no antibacterial activity against S. aureus and S. typhi (p-values

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