Antibacterial activity of Trigona biroi (Philippine stingless bee) propolis on Escherichia coli and Staphylococcus aureus

Date of Completion


Document Type


Degree Name

Grade 12


Propolis, Trigona biroi, Escherichia coli, Staphylococcus aureus, Zone of Inhibition


Propolis’ are bee products that are found to possess antibacterial activity. However, propolis from Trigona biroi, endemic specie of stingless bee, has limited studies regarding its benefits. As considered, propolis products are not utilized to the maximum extent. The aim of this study is to assess the antimicrobial activity and determine the zone of inhibition of propolis extract from Trigona biroi on certain bacterial species. The antimicrobial activity of propolis extracts in three different concentrations and two antibiotic drugs was determined in through Kirby Bauer Disk Diffusion method. These samples were subjected on Staphylococcus aureus and Escherichia coli to determine the sensitivity of these bacteria to the tested samples. Zone of inhibitation were compared with that of different standards with antibiotic drugs particularly 5μg Ciprofloxacin and 30μg Vancomycin. To stipulate whether the three concentrations have significant differences in terms with the inhibition, One-way Analysis of Variance (ANOVA) (p = 0.05) was used. A follow-up Tukey’s HSD Test was used to distinguish which concentrations are significantly different between each other. The results indicate that the 100-mg/ml concentration of ethanolic extract of propolis (EEP) has a partially active antibacterial activity on E. coli, but were inactive on S. aureus. Moreover, the antibacterial activity of 5μg Ciprofloxacin and 30μg Vancomycin are more efficacious to inhibit S. aures than the three prepared concentrations. However, the test on E. coli indicates that only the 5μg Ciprofloxacin was the only sample among the rest that had a very high antibacterial activity, while other samples recorded an inactive antibacterial activity.

First Advisor

Teresita T. Pineda

This document is currently not available here.