Title

Detection of pathogenic E. coli from commonly eaten raw vegetables sold in local markets and supermarkets in the City of Dasmarinas, Cavite

Date of Completion

7-2019

Document Type

Thesis

Degree Name

Grade 12

Keywords

Escherichia coli, raw vegetables, local markets, supermarkets, Raw Foods

Abstract

This study is about the detection of pathogenic E.coli in commonly eaten raw vegetables. Pathogenic bacteria are invasive bacteria that can cause diseases when they enter a human body. E.coli O157:H7 is a type of pathogenic bacteria that could cause diseases when they enter the human body. Eating vegetables provides many nutrients to the human body but they could also be the sources of diseases if the vegetables are contaminated by a pathogenic bacteria like E.coli if not prepared properly. The researchers used 3 different types of vegetables that are obtained from the local wet markets and supermarkets. The vegetables then underwent enrichment, serial dilution, plating, and incubation. The results of the data gathered during the experiment shows that nearly half of the vegetables samples contained pathogenic bacteria that is not only limited by E.coli, thereby confirming its presence. This implies that these vegetables were infested with bacteria upon purchase, including those bought from supermarket and it could potentially affect humans upon consumption of it. In conclusion, these vegetables may pose a harm to one’s nutrition, if not prepared properly.

First Advisor

Myra Michelle M. Mojica

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