Title

Quantitative estimation of total phenolics and flavonoids and analysis of the antioxidant activity of the ethanolic extracts of paragis (Eleusine indica)

Date of Completion

6-2019

Document Type

Thesis

Degree Name

Grade 12

Keywords

Paragis, Plant Extracts, Eleusine indica, Antioxidant property, Phenols, Flavonoids

Abstract

This study aims to quantify the total phenolic content and total flavonoid content, analyze the antioxidant activity of the Paragis (Elusine indica) ethanolic crude leaf extract and compare them to species of the same family (Poaceae). Quantitative analysis was done, as the study was experimental by nature, but utilized the descriptive research design. Total phenolic content (TPC) was determined using the Folin-Ciocalteau reagent method, while the total flavonoid content (TFC) was determined using the Aluminum Chloride Colorimetric Method. Antioxidant activity was analyzed using the 2,2-diphenyl-l-picrylhydrazyl (DPPH) free radical scavenging asasy. To calrify, each parameter utilized a spectrophotometer and concentrations were measured at certain wavelengths to crease calibration curves and absorbances which involved regression analyses. Results exhibited that E. indica extracts contain TPC of 151.49 mg/L in gallic acid equivalents and TFC of 140.86 mg/L in quercitin equivalents. Antioxidants activity was interpreted using IC values having 1053.38 mg/L as the concentration inhibiting 50% of the free radical. The antioxidant activity of the extracts was mainly influenced by plant metabolites which are phenols and flavonoids, and were investigated through their IPC and TFC. Direct relationship between concentrations and absorbance were reported. Triplicate analyses within parameters are recommended to be employed for accurate results. Furthermore, it is recommended that antioxidant activity of E. indica should be studied in line with other pharmacological benefits as well as considering the part of the plant to analyze and to comprehensively compare these properties to species in the same family.

First Advisor

Mark Lawrence F. Fabregas

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