Title

Detection of Escherichia coli on sunny brooke I dam in General Trias Cavite

Date of Completion

6-2019

Document Type

Thesis

Degree Name

Grade 12

Keywords

Escherichia coli, bacteria, Sunny Brooke Dam, Dam, Water Contamination

Abstract

Inappropriate disposal of trash that has been a problem, not only in urban areas but also in rural regions. This problem has caused negatively to the quality of water which, in the end, has been the presence of disease causing bacteria. The most common of them is the Escherichia Coli or E. Coli which affect the intestinal and urinary tract. People who consume contaminated water or food full of these bacteria may lead to poisoning. Thus, the objectives of this study is to determine the presence of Escherichia coli in Sunny Brooke Dam in General Trias Cavite., to identify the significant number of Escherichia coli in the said dam and to determine the safety measures the people have been practicing before using it for daily activities. Gathering of data was done in Sunny Brooke I dam. The detection of Escherichia coli was performed in the laboratory which necessitates serial dilution and multiple tube fermentation technique. The three sections of multiple fermentation technique are composed of presumptive test, confirmed test, and the completed test. The Colony-Forming units and Most Probable Number were used to interpret the results of the protocol. Major findings of the study involved the presence of E. coli and the dam contains high fecal coliform which is indicated through the MPN that is greater than 1100. This means that the dam is contaminated and requires thorough cleaning.

First Advisor

Pauline L. Cueno

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