Date of Completion


Document Type

Research Project

Degree Name

Grade 12


staphylococcus aureus, escherichia coli, fast food, utensil, disposable spoons


Majority of people dine or eat in fast food chains which gives them the option to choose what utensil to use: Stainless steel or Disposable utensils. Staphylococcus aureus can remain viable on hands and environmental surfaces for extended durations after initial contact. Escherichia Coli are mostly harmless bacteria however some may cause infections when transmitted directly or indirectly. Six disposable spoons from selected fast food chains along Dasmariñas City, Cavite were obtained to identify the presence or absence of the bacteria S. aureus and E. coli. The results are determined on the standards for number of spoilage microorganisms on food contact based from the Compendium of Methods for the Microbiological Examination of Foods, 5th Edition. Although, the researchers’ experience in acquiring the samples were different from different fast food chain, results obtained show that all disposable spoons tested resulted to an excellent remark by having a less than 1 CFU/cm^2 for Staphylococcus Aureus and an absence of Escherichia Coli. It is deemed that disposable spoons is safe to use because of low microbial count.

First Advisor

Mark Lawrence F. Fabregas