Title

Bacterial detection on fresh and frozen meat samples marketed in the City of Imus, Cavite Philippines

Date of Completion

7-2019

Document Type

Thesis

Degree Name

Grade 12

Keywords

Fresh Meat, Foodborne Diseases, Food Contamination, Frozen Meat, CFU, Colony Forming Units, local market

Abstract

Foodborne illnesses are a worldwide concern up to date. These are diseases that infect the gastrointestinal tract due to the invasion of bacteria, parasites and virus. Food such as raw meat are highly associated with foodborne illness as some harmful bacteria are already present in it due to high moisture content and nitrogenous compounds. This study aimed to detect the presence of bacteria in fresh and frozen minced meat (ground pork) sold in local markets along Imus City, Cavite and determine if there is a significant difference between the number of colony forming units (CFU) present between the fresh and the frozen minced meat (ground pork) sold in local markets along Imus City, Cavite. Furthermore, it aimed to determine if the environmental conditions of the markets contribute to the bacterial population. For the meat sample, a ten-fold serial dilution was conducted and transferred to nutrient agar plates. The plates were incubated at 37 degrees Celsius for 24 hours and colony forming units (CFU) were computed to measure of variable bacterial or fungal cells. The fresh minced meat (ground pork) samples resulted in high colony forming units, while frozen minced meat (ground pork) samples have a few colony forming units. However, based on the independent t-test, both samples are not significantly different. Also, the environmental conditions used was the clearance requirements of the wet markets and supermarkets and it contribute to bacterial growth.

First Advisor

Pauline L. Cueno

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