Date of Completion


Document Type

Research Project

Degree Name

Grade 12


Angiogenesis, ethanolic leaf exract, Jatropha curcas, Chorioallantoic Membrane (CAM), Blood vessels, Duck Embryo


With various uses and benefits, plants are widely utilized in the Philippines. Jatropha curcas (Tuba-tuba) plant is among the popular plants believed to give medicinal benefits. In line with this, such plants may be identified and experimented on for its possible inhibitition or stimulation of angiogenesis. This study aimed (1) to determine the significant effect of varying treatments injected to the CAM, and (2) to identify the effects of the variation of concentrations. Experimental method was used as the research method wherein the main subjects were the 28 duck eggs selected randomly, Jatropha curcas ethanolic leaf extract with three varying concentrations (100 ppm, 200 ppm, 300 ppm) diluted in Normal Saline Solution (NSS), and a negative control treatment. After 48 hours of incubation of duck eggs after administration of treatments, major blood collaterals were manually counted. Upon observation, results showed that T1 (100 ppm) and T3 (300 ppm), inhibited the number of blood vessels, whereas the T2 (200 ppm) increased the major collateral count. Based from the statistical analysis, T1 was the most effective treatment in inhibiting the number of blood vessels. Therefore, Jatropha curcas ethanolic leaf extract is an inhibitor of angiogenesis where it has a possibility to be used for treating tumor or cancer.

First Advisor

Pauline L. Cueno