Antibacterial effect of Mimosa pudica (Makahiya) and Kalanchoe pinnata (Kataka-taka) ethanolic leaf extracts against Staphyloccocus aureus

Date of Completion


Document Type

Research Project

Degree Name

Grade 12


Mimosa pudica, Kalanchoe pinnata, Staphylococcus Aureus, Zone of Inhibition, Antibacterial


Kalanchoe pinnata, or Kataka-taka, and Mimosa pudica, or Makahiya, are two plants commonly found in the Philippines that are often used to treat inflammatory and infectious processes. These plants are to be used as the main constituents of our study. This study aimed to gauge the antibacterial activity of the ethanolic extract of Kataka-taka and Makahiya. To verify its antibacterial activity, Staphylococcus Aureus was chosen to be the bacteria of the study due to previous research finding its prevalence in human hands, a common site of transmission for bacteria. Kataka-taka and Makahiya leaves were oven-dried for 1 week and 3 days, respectively. The dried leaves were then blended and macerated with ethanol to obtain a solution filtered using a rotary evaporator to acquire the pure extract. Kirby Bauer Disk Diffusion was used for the antibacterial screening, with Vancomycin and Ethanol as our positive and negative control. Different concentrations (100%, 75%, and 50%) of the plants’ ethanolic extract were tested by using this method. In evaluating the antibacterial activity of the extracts of the two plants, M. pudica was found to be more effective due to its significant Zones of Inhibition (ranging from 7mm to 17mm) against S. Aureus. This study supported the notion that M. pudica has significant antibacterial properties, supporting its case as a plant that can be used in traditional medicine. The plant could be used as a potential antibacterial product, such as a hand sanitizer.

First Advisor

Myra Michelle M. Mojica

This document is currently not available here.