Date of Completion


Document Type


Degree Name

Grade 12


Bacteria, microbial colonies, toilet seats


Microorganisms are small living organisms that cannot be seen by the naked eye but can be found anywhere in the environment. One of these microorganisms are pathogens which can affect the health of a living organism through various ways. Furthermore, these microorganisms can be transferred through direct or indirect transmission which relates into toilet seats that act as a vehicle for the transmission of pathogens from one person to another. With that, the study was conducted to determine the prevalence of microbial organisms such as bacteria and fungi in toilet seats of Lourdes E. Campos Building of De La Salle Medical and Health Sciences Institute. Also, the study aims to classify the bacterial organisms present into gram-positive and gram-negative bacteria. Ten samples were collected in different restrooms along the Lourdes E. Campos Building and each sample is divided into three dilution factors. The samples were subjected for the process of colony counting and gram staining. Out of all samples collected, most of them shows that there are more than 300 bacterial colonies on these toilet seats which some of them also contains another microorganism called fungi. On the other hand, significantly almost all samples were classified as a bacterium with a gram-negative classification. Data shows that toilet seats contain microbial organism which can create potential health problems with people using them.

First Advisor

Carmellete S. Davila